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qPCR: The Very Basics
Real-Time (or quantitative, qPCR) monitors PCR amplification as it happens and allows you to measure starting material in your reaction. Data are presented graphically rather than as bands on a gel....
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Moving Towards Zero Hunger, One Genome at a Time
Farmer and a pile of cassava bulbs. Have you ever thought about plant viruses? Unless you’re a farmer or avid gardener, probably not. And yet, for many people the battle against agricultural viruses...
View ArticleA Quick Method for A Tailing PCR Products
Some thermostable DNA polymerases, including Taq, add a single nucleotide base extension to the 3′ end of amplified DNA fragments. These polymerases usually add an adenine, leaving an “A” overhang....
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Cloning Blunt-Ended DNA Fragments is Hard: pGEM®-T Vectors Can Make It Easier.
PCR amplification with a proofreading polymerase, like Pfu DNA polymerase, will leave you with a blunt end. However, another thermostable DNA polymerase, like Taq DNA Polymerase, adds a single...
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Researching the Researchers: Aberto Biscontin
2019 Real-Time PCR Grant The three 2019 Real-Time PCR Grant Winners have been hard at work in the six months since winning their grants. Each winner was eligible to receive up to $10,000 in free PCR...
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Researching the Researcher: Abbeah Navasca, 2019 Real-Time PCR Grant Winner
The three winners of the 2019 Real-Time PCR Grants have been hard at work in the six months since receiving their grants. Each winner was eligible to receive up to $10,000 in free PCR reagents as well...
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XpressAmp™ Direct Amplification: Simplified and Accelerated Time to qPCR Results
As the SARS‐CoV‐2 pandemic continues to rage across the United States and around the globe, the demand for COVID‐19 testing is increasing. The vast majority of the COVID-19 assays use RT‐qPCR to...
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There’s a Microbiome In My Tank!
Imagine a scenario—you’re studying the developmental biology of a species of squid. The squid don’t reproduce in captivity, so females carrying fertilized eggs are collected from the wild and rehomed...
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An Introduction to Lyophilization: Process, Benefits & Possibilities
Lyophilization is a process designed to remove water from a sample or product through a controlled freezing and vacuum application. The method leverages the triple point of water, where solid, liquid,...
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Understanding and Combating Legionella in Water Systems with Viability PCR
Water plays a vital role in countless aspects of daily life—drinking, cooling, recreation and more. However, the same systems that deliver these benefits can also harbor Legionella, a waterborne...
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